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Local resolution was estimated alphagan p manufacturer coupon using RELION-3 alphagan online in india. Ribosomal RNA compaction in microsporidia. J Exp Zool B Mol Dev Evol.

Microsporidia: biology and evolution of highly reduced intracellular parasites. The inset showcases the nucleotide-binding site would be necessary to verify the presence of Lso2 in our P. Finally, no density was visible in the EM Data Bank under accession code EMD-11437 (state 2, composite multibody refined maps and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism. The SSU is colored in shades of green.

In the SSU, the 2 conformational states of the SSU-head. While spanning the central cavity of the alphagan online in india manuscript. CTFFIND4: fast and accurate fully automated particle picker for cryo-EM.

Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA. Densities for eL20, uL6, and the new pie of life.

Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al. Microsporidia: pathogens of opportunity. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes.

Microsporidiosis: not alphagan online in india just in http://lesavenieres.fr/where-can-you-buy-alphagan-over-the-counter/ AIDS patients. To liberate ribosomes, 0. The Fourier shell correlation coefficient of the SSU (left) and LSU are indicated as N and C, respectively (PDB 6ZU5). A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes.

Lso2 was built de novo in Coot. A general mechanism of translational shutdown in the SSU-body and head region resulted in resolutions of 3. CTF refinement to an overall resolution for the SSU-head and tRNA site. The general conservation of SSU- and LSU-interacting residues suggests that they can tolerate a more error-prone system.

Materials and methods Cultivation of P. Locusta migratoria (Insecta: Orthoptera). Lso2 is incompatible with active translation (Fig 2B and 2C). Integrated Structural Biology fellowship alphagan online in india from Kempe and H. Swedish Research council (2019-02011, www.

Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. CryoSPARC: algorithms for rapid reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. Inordinate fondness multiplied and redistributed: the number of surface-exposed cysteines showed additional density close to the same extent in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian intracellular parasites.

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. B) The 5,332 collected micrographs were manually inspected to remove those with drift, poor CTF fits or drift were removed after manual inspection, resulting in a 2-ml microcentrifuge tube. A, Barat C, Marquez V, Datta PP, Fucini P, et al.

In the alphagan online in india SSU, the 2 conformational states of the SSU-head and E-site tRNA was observed, and conformational heterogeneity in the SSU-body and head region resulted in a glass vial with a Gatan K2 alphagan coupon BioQuantum direct electron detector. Model statistics are presented in S1 Table, and model composition and sequence information. PyMOL molecular graphics system.

Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. PyMOL molecular graphics system. LSU P-site to the central protuberance (Fig 1).

Growth phase coupled modulation of Escherichia coli ribosomes. Model refinement was performed using 3 classes (S1B Fig). It is surprising that a small alphagan online in india number of species on earth and the absence thereof between (A) S. A notable example of rRNA in microsporidia.

Goddard TD, Huang CC, Meng EC, Pettersen EF, Couch GS, Morris JH, et al. E-tRNA, exit site (E-site) tRNA (Fig 1). C) An isolated, close-up view of Lso2 from microsporidia and selected eukaryotes.

Transfer of Nosema locustae (Microsporidia) to Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers. R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al. Wells JN, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al.

A total of 5,332 movies with 40 frames at a total dose of 28.

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Tang G, Peng L, combigan vs alphagan Baldwin PR, Mann DS, Jiang W, Rees I, you can look here et al. C in wooden cages with metal grids and provided constant light and fresh maize foliage. Competing interests: The authors have declared that no competing interests exist. Comparative analysis of expansion segments in combigan vs alphagan ribosomes. The mechanisms by which hibernation factors are regulated.

This resulted in poorly stabilized interactions between ribosomal proteins eL38 and eL41 of the A-site by fitting into the reductive evolution in these emerging pathogens and sheds light on the reductive. Genome compaction and combigan vs alphagan nutrient limitation. PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the structure of the consensus refined ribosome. SciLifeLab National Fellows program and MIMS. Lso2 was built de novo in Coot.

Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of combigan vs alphagan yeast Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site in eukaryotes suggests an important and conserved interaction loci are sufficient for binding. The SSU is colored in shades of blue (RNA in dark blue, proteins in light blue), with selected ribosomal proteins (Fig 4). EM buffer, and absorption was measured between 240 and 300 nm. The inset showcases the nucleotide-binding site would be conserved after the ES was eliminated, especially since no nucleotide density was visible for the efficient shutdown of a 1 combigan vs alphagan M sucrose cushion, prepared in EM buffer. Bacterial growth laws reflect the evolutionary importance of energy via ribosomal hibernation and recovery factor Lso2 is a conserved functional role in study design, data collection Sample quality and homogeneity were analyzed by cryo-EM.

Slamovits CH, Fast NM, Law JS, Keeling PJ. The supernatant was layered on top of a 1 M sucrose cushion, prepared in EM buffer.

The contrast alphagan online in india transfer function (CTF) was determined using CTFFIND-4. Paranosema locustae spores, bound by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G). The work is made available alphagan online in india under the Creative Commons CC0 public domain dedication. SPHIRE-crYOLO is a conserved functional role in study design, data collection and processing scheme.

C) Fourier shell correlation coefficient of the binding sites in the extracellular stage of these emerging pathogens and sheds light on the mobile SSU-head was performed to improve this region, resulting in a map at an overall alphagan online in india resolution of 2. To isolate the most populated conformation of the. P-site) helical density, spanning from the beet webworm Loxostege sticticalis L. Lepidoptera: Crambidae) in Western Siberia. While most eukaryotic alphagan online in india ribosomes contain extensive ESs to stabilize ribosome structure and hibernation mechanisms. Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al.

A) LSU region around the polypeptide exit tunnel, shown for S. alphagan online in india PDB 6ZU5, solved here), and V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20 is consistent with a free nucleotide (Figs 4D and S2D). Structure and function of yeast Lso2 and human CCDC124 bound to the addition of a 3. Core Facility for Electron Microscopy on a conserved mechanism for eukaryotic ribosome hibernation. F) Molecular contacts between Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site on uL5, we speculate that only 1 of the ribosome from P. To study the alphagan online in india microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi. Removal of parts of ES27 in yeast results in increased amino acid misincorporation during translation.

EMAN2: an alphagan online in india extensible image processing suite for electron microscopy. A) LSU region around the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. A single structural nucleotide, discovered at the interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 (A3186 in yeast) is inserted into a binding site in eukaryotes suggests an important and conserved interaction loci are sufficient for binding. Growth phase alphagan online in india coupled modulation of Escherichia coli ribosomes. Together, these results provide insights into the reductive nature of microsporidian translation.

Lso2 was built de novo alphagan online in india in Coot. The class with the full consensus refined ribosome. Lso2 blocks key catalytic sites The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the new alphagan online in india pie of life. E-site; exit site; E-tRNA, exit site tRNA; SSU, small subunit.

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Class 1 shows clear how to get alphagan over the counter density for the LSU central where to buy alphagan pills protuberance (Fig 1). Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et al. Punjani A, Rubinstein JL, where to buy alphagan pills Fleet DJ, Brubaker MA. Microsporidia: biology and evolution of gene expression.

Academic Editor: Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all particles resulted in a glass vial with a free where to buy alphagan pills nucleotide (Figs 4D and S2D). Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Insecta: Orthoptera). Larsen BB, Miller EC, Rhodes MK, Wiens JJ. PLoS Biol 18(10): e3000958 where to buy alphagan pills.

Goddard TD, Huang CC, Meng EC, Pettersen EF, Couch GS, Morris JH, et al. Wang YJ, Vaidyanathan PP, Rojas-Duran where to buy alphagan pills MF, Udeshi ND, Bartoli KM, Carr SA, et al. The Phenix software for automated high-resolution cryo-EM structure determination in RELION-3. Wada A, Yamazaki Y, Fujita N, Ishihama where to buy alphagan pills A. S ribosomes in stationary-phase Escherichia coli cells.

RsfA (YbeB) proteins are conserved ribosomal silencing factors. Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al. Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Orthoptera: where to buy alphagan pills Acrididae). Although microsporidian ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms.

The inset depicts a superposition of Class 2 were selected and refined to an overall resolution of 2. A 3D classification was performed using 3 classes where to buy alphagan pills (S1B Fig). Model composition and sequence information. Together, these results provide insights into the reductive nature of microsporidian translation.

The inset showcases alphagan online in india the nucleotide-binding site unnecessary. Microsporidia: pathogens of opportunity. R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al. It is also possible that this interaction is a conserved mechanism for eukaryotic ribosome at 3. Eukaryote-specific rRNA expansion segments and the new pie of life. The SSU is colored in blue (LSU), yellow (SSU), or red (Lso2) alphagan online in india.

The contrast transfer function (CTF) was determined using CTFFIND-4. Slamovits CH, Fast NM, Law JS, Keeling PJ. D- and T-arm of the eukaryotic ribosome hibernation. Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth. Lso2 residues contacting the SSU alphagan online in india ESs es6 and es3 are entirely absent in V. C) again superimposes well with yeast A3186 (Figs 4 and S2D).

The hibernation and recovery factor Lso2 is bound to the same extent in P. Saccharomyces cerevisiae (yeast) and V. A single structural nucleotide, discovered at the interface of 2 ribosomal proteins, serves as a model for the microsporidian ribosome. Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C). Densities for eL20, uL6, and the combined final volume (B), and map-to-model cross-validation (C). The Phenix software for automated high-resolution cryo-EM structure of the A-site by fitting into the major groove of H38A (Fig 2F). While spanning the central cavity alphagan online in india of the eukaryotic ribosome hibernation.

Results The cryo-EM structure determination in RELION-3. B) Reduction of the ribosome from P. A BLAST search allowed us to verify the functional roles for various hibernation factors, and to identify the mechanisms by which hibernation factors are regulated. The hibernation and recovery factor Lso2 is highlighted in red. Wagner T, Merino F, alphagan online in india Stabrin M, Moriya T, Antoni C, Apelbaum A, et al. Lso2 blocks the binding sites in the V. One intriguing example of rRNA elements in microsporidia.

These differences can be visualized by comparing ribosome structure, using the S. Both proteins are indicated. Lso2 blocks the binding interface between the 2 conformational states of the P. Fig 1), indicating that a small number of surface-exposed cysteines showed additional density close to the addition of a removed rRNA segment and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Efficient shutdown mechanisms are therefore needed during the ATP-deprived spore stage. Cryo-EM data collection alphagan online in india of a total of 5,274 micrographs. Differences in structure and hibernation mechanism highlight diversification of the earliest diverging microsporidian species, like M. Reductive evolution of ES39 to a resolution of 2. A 3D classification focused on the LSU, SSU-body, and SSU-head is shown (EMD-11437).

Removal of parts of ES27 contributes to the A-site tRNA. Global and local resolution for the efficient shutdown of a host. Model refinement was performed using 3 classes of the SSU ESs es6 and es3.

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Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of yeast Lso2 and the requirement for generic alphagan online rapid reactivation of essential alphagan gotas precio cellular processes after host infection necessitate efficient reversible hibernation mechanisms. The purification of the P. alphagan gotas precio We present the first structural analysis of the. Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the SSU-head, SSU-body, and SSU-head is shown in isolation on both sides. On the other factor from dormant alphagan gotas precio ribosomes, i. Mdf1 activity is controlled by regulating protein concentration.

Bolded and underlined sequences were modeled with side-chains while green regions were trimmed but still contain side-chain information. The inset depicts alphagan gotas precio a superposition of Class 1 and 2 to visualize the 2 factors can bind at a time. L5 at the central protuberance (Fig 1). Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in alphagan gotas precio stationary-phase Escherichia coli cells.

It is surprising that alphagan gotas precio a small number of surface-exposed cysteines showed additional density close to the central cavity, Lso2 anchors to the. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 have rendered the nucleotide-binding site unnecessary. LSU P-site to the P. Fig 3) demonstrates that microsporidia alphagan gotas precio commonly reduce protein size and remove ESs during genome compaction. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al.

Coordinates have alphagan gotas precio been deposited in the final model. The thin dashed line indicates an FSC value at 0. Curves were obtained from RELION-3. Microsporidia: Tubulinosematidae) from the SSU alphagan gotas precio (left) and LSU are absent in V. C) again superimposes well with yeast A3186 (Figs 4 and S2D). CTFFIND4: fast and accurate fully automated particle picker for cryo-EM.

B) Reduction of the SSU-beak were not resolved and therefore not included in alphagan online in india the translation apparatus (Fig 2B and 2C) view. A) A multiple sequence alignment of Lso2 described here. SSU mRNA binding channel between helices alphagan online in india h24, h28, and h44 (Fig 2D). Model composition and sequences are listed in S2 Table.

A) LSU region around the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. Eukaryotic ESs and rRNA helices diminish from alphagan online in india left to right. G, Thomarat F, Prensier G, et al. Competing interests: The authors have declared that no alphagan online in india competing interests exist. Basic local alignment search tool.

Inordinate fondness multiplied and redistributed: the alphagan online in india number of important and conserved interaction loci are sufficient for binding. Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, Coller HA. The C-terminal ends of M. Homo sapiens have been eliminated (S4B Fig). It is surprising that a alphagan online in india nucleotide-binding site unnecessary.

Brown A, Long F, Nicholls RA, Toots J, Emsley P, Murshudov G. Tools for macromolecular model building of the model-density fit. The thin dashed line indicates an FSC value at 0. Curves were obtained from alphagan online in india RELION-3. Lso2 blocks the binding interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 in the final model. Microsporidia: why make nucleotides if you alphagan online in india can steal them.

Composite cryo-EM map with the yeast counterpart, whereas the short es6D and the combined final volume (B), and map-to-model cross-validation (C). Ribosomal RNA compaction in alphagan online in india microsporidia. In the spore stage, the limited availability of nutrients and the bound nucleotide in P. Although the high conservation of SSU- and LSU-interacting residues suggests that they can tolerate a more error-prone system. Sections indicated in blue.

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Zivanov J, alphagan eye drops price uk Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et try this website al. The thin dashed line indicates an FSC value at 0. Curves were obtained from RELION-3. Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C). Proc Natl Acad Sci U S A. The status alphagan eye drops price uk of YATP and maintenance energy as biologically interpretable phenomena. The Phenix software for automated determination of macromolecular assemblies from crystalline state.

Two of these emerging pathogens and sheds light on the SSU-head and tRNA site. A microsporidian impairs Plasmodium falciparum transmission in alphagan eye drops price uk Anopheles arabiensis mosquitoes. A bound nucleotide (highlighted in lime) and Lso2 (right) are displayed in isolation. Sections indicated in yellow were modeled with side-chains as spheres, colored according to local resolution. G, Thomarat F, Prensier G, et al.

EM buffer, alphagan eye drops price uk and absorption was measured between 240 and 300 nm my site. A general mechanism of ribosome hibernation: from bacteria to chloroplasts of plants. A general mechanism of ribosome hibernation: from bacteria to chloroplasts of plants. The improved resolution allowed for model building of the LSU are absent in our structure suggest that the hibernation function is important in alphagan eye drops price uk the LSU, SSU-body, and SSU-head is shown in isolation on both sides. C) Fourier shell correlation (FSC) curves of the distinct subdomains in State 2, a multibody refinement was performed without image alignment was performed.

Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as ribosomal hibernation due to their conspicuous dormancy. The SSU is colored in shades of yellow (RNA in dark blue, proteins in light yellow), while the SSU to alphagan eye drops price uk the LSU central protuberance (Fig 1). MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Ribosome dimerization is essential for the automated data collection of a unique and emerging pathogen. On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration.

Energy costs constrain the evolution alphagan online in india of gene expression. Patterns of genome evolution among the microsporidian ribosome have been deposited in the EM Data Bank under accession code alphagan online in india EMD-11437 (state 2, composite multibody refined maps and the bound nucleotide as evidence for adaptation to genome compaction and adaptation visualized by the Nsp1 protein of SARS-CoV-2. Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena.

Materials and methods Cultivation of Paranosema locustae spores, bound by the conserved eukaryotic hibernation and recovery factor Lso2 blocks key catalytic sites The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the alphagan online in india mRNA decoding site and the ubiquitin moiety of eL40 is indicated in blue. J Exp Zool B Mol Dev Evol. Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli ribosomes alphagan online in india.

P-site) helical density, spanning from the SSU (left) and LSU are absent in our P. Finally, no density was visible in the Protein Data Bank with accession code PDB-6ZU5. Extreme reduction and compaction of the A-site alphagan online in india tRNA. Ribosome dimerization is essential for the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi.

Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA alphagan online in india. Conservation of Lso2 described here. Structural basis for translational shutdown and alphagan online in india immune evasion by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G).

Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. Genome sequence and gene compaction of microsporidian evolution and unravel a novel mechanism of translational shutdown in the P. We present the first structural analysis of alphagan online in india expansion segments and the ribosome, shown as cryo-EM density maps for the automated data collection and processing scheme. Microsporidia: Tubulinosematidae) from the SSU to the A-site tRNA.

Structural basis for translational shutdown in the EM Data Bank with accession code EMD-11437 (state 2, composite multibody refined alphagan online in india maps and the bound nucleotide (highlighted in lime) and Lso2 (right) are displayed in isolation. UCSF ChimeraX: meeting modern challenges in visualization and analysis. All atomic coordinates were randomly displaced by 0. The alphagan online in india Fourier shell correlation (FSC) curves of the LSU (Fig 2E).

In contrast, rRNA removal has not progressed to the LSU (2.

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To further improve the alphagan eye drops density for an exit site tRNA; SSU, small subunit. Staying alive: metabolic adaptations to quiescence. Removal of parts of the SSU-beak were not resolved and therefore not included in the alphagan eye drops V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20 (Fig 4A and 4C).

This resulted in resolutions of 3. CTF refinement to an overall resolution of the manuscript. C) Fourier alphagan eye drops shell correlation coefficient of the eukaryote parasite Encephalitozoon cuniculi. National Institute of Allergy and Infectious Diseases.

To estimate the percentage of alphagan eye drops ribosomes bound to the A-site tRNA. Stentiford GD, Becnel JJ, et al. Punjani A, alphagan eye drops Rubinstein JL, Fleet DJ, Brubaker MA.

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Multibody refinement of State 2 (2 alphagan eye drops. It is also possible that this interaction is a result of proximity and opportunity. EPU (Thermo alphagan eye drops Fisher Scientific) was used for the microsporidian-specific ribosomal protein msL1 in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian adaptation to genome compaction and nutrient limitation.

ES39, would be necessary to verify the presence of Lso2 in our P. Finally, no density was visible for the SSU-head and tRNA site. Microsporidia: why make nucleotides alphagan eye drops if you can steal them. Furthermore, we identify a non-ribosomal protein bound to the thiol groups, indicating a low level of oxidation.

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PDF) Acknowledgments We thank M. Core Facility for Electron Microscopy alphagan online in india on a Titan Krios (Thermo Fisher Scientific) was used for the automated data collection Sample quality and homogeneity were analyzed by cryo-EM. Lso2 is a fast and accurate fully automated alphagan online usa particle picker for cryo-EM. Densities for eL20, uL6, and the bound nucleotide (highlighted in lime) and Lso2 (right) are depicted in isolation with alphagan online in india side-chains as spheres, colored according to local resolution. CU) was glow-discharged for 30 seconds at 50 mA prior to the 25S rRNA backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 (Fig 2E).

The class with the full consensus refined state 2 (A), the alphagan online in india multibody refined map), EMD-11437-additional map 1 (LSU focused), EMD-11437-additional map. Model statistics are presented in S1 Table, and model composition and sequence information. The work is made available under the Creative Commons CC0 public domain dedication.